recG [2017-8-11 16:0:3]

ATP-dependent DNA helicase, branch migration translocase, required for DNA repair and chromosomal segregation

locus: BSU15870

pI: 7.09

mw: 77.96 kDa

protein length: 682 aa

gene length: 2046 bp

function: DNA repair and chromosomal segregation

product: ATP-dependent DNA helicase, branch migration translocase

essential: no

synonyms: ylpB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

Gene

Coordinates: 1,659,810 → 1,661,858

Phenotypes of a mutant

• a [gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2] [gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG] double mutant is not viable [pubmed|28527403]

The protein

Catalyzed reaction/ biological activity

• binds and unwinds Holliday junction DNA [Pubmed|24770420]

Protein family

• helicase C-terminal domain (according to Swiss-Prot)

Paralogous protein(s)

• [protein|AD1F39823ABCE2A222259B879513752942C59590|Mfd]

Structure

• [PDB|1GM5] (from ''Thermotoga maritima'', 43% identity, 62% similarity)

Expression and Regulation

Operons

• genes: [gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]-[gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA]-[gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG]
  description: [Pubmed|22383849]

Biological materials

Mutant

• 1A897 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A897&Search=1A897 BGSC]

References

• 17853894,15533834,17640277,24770420,21170359,28527403